Using a microscope is like seeing the invisible by your own naked eyes. That’s the wonder that only a microscope can provide. And speaking of a microscope, phase contrast microscopes are the only microscope that does not require staining to view the slide that made it possible to study the entire cell cycle. This equipment utilizes a special viewing technique that translates a shift in light wave phase to a shift in intensity, and thus visible contrast. Phase contrast microscopes are the most common equipment for examining such specimens as biological tissues. This a type of light microscopy that detects every detail of living cells since phase contrast microscope that enhances contrast of transparent and colorless objects by influencing the optical path of light. This is the only microscopy equipment that uses the fact that the light passing through a transparent part of the specimen travels a slower phase. Due to this, the light shifted to the uninfluenced light. According to a research, the human eye measures only the energy of light arriving on the retina, so changes in phase are not easily observed; yet often these changes in phase carry a large amount of information.

Microscopes for phase contrast all started in the year 1930’s when it was invented by a Dutch physicist Fritz Zernike, who became a Nobel Prize recipient for physics in 1953 for his invention of the phase contrast microscope. Based on a study made by Zernike, phase contrast microscopy technique can be used to optical microscopy. It was during his studies that he appreciated both that is necessary to interfere with a reference beam, and that is to maximize the contrast achieved with the technique. And it is necessary to introduce a phase shift to this reference so that the no-phase-change condition gives rise to completely destructive interference. The phase contrast method is now a vital and invaluable technique when photographing or filming living cells in action. Now, phase contrast microscopes optical technique are now used to produce high contrast images of transparent specimens, such as living cells, thin tissue slices, microorganisms, lithographic patterns, latex dispersions, fibers glass fragments, and that includes nuclei and other organelles.